By Karl Maramorosch
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Additional resources for Invertebrate Cell Culture Applications
In practice, the culture period should be limited to one generation time and the measured values of the changes divided by the initial number of cells. To do such experiments synchronous cell cultures have to be used. Such experiments may be possible, although many difficulties may be encountered. Figures 1 to 21 are based on data obtained from cultures which were performed under different conditions. It is, therefore, meaningless to discuss minor differences observed in those patterns of amino acid changes.
Arginine is thought to be important as a member of the ornithine cycle. However, the presence of this cycle is not well known in insects, especially in insect cells in cultures. Asparagine The omission of asparagine from the culture medium has little effect on the growth of lepidopteran cell lines, in spite of the fact that these cell lines consume asparagine in considerable amounts. Mosquito cell lines also consume a s paragine in considerable amounts. Their growth rates decrease when asparagine is omitted.
The cells used for experimental cultures were washed with saline before placing them in test media. However, the effects of carry over amino acids cannot be denied. Long-term experiments with serial passages were then attempted by using amino acid-deficient media. Subculturing was p e r formed at least once a week with split ratio of 1 to 2. The amino acids of which a deficiency did not support the subculture more than 10 passages were considered to be essential amino acids for the growth of the cells (Fig.