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Download Cells and Tissues in Culture Methods, Biology and Physiology by E. N. Willmer PDF

By E. N. Willmer

Cells and Tissues in tradition: tools, Biology, and body structure, quantity three specializes in the purposes of the equipment of tissue tradition to numerous fields of research, together with virology, immunology, and preventive medication.
The choice first deals info on molecular association of cells and tissues in tradition and tissue tradition in radiobiology. issues comprise mobile association on the molecular point, fibrogenesis in tissue tradition, impact of radiation at the development of remoted cells, and irradiation of the chosen elements of the telephone. The booklet then considers the results of invading organisms on cells and tissues in tradition and mobilephone, tissue, and organ cultures in virus learn.
The e-book elaborates on antibody construction in tissue tradition and tissue tradition in pharmacology. Discussions specialize in early makes an attempt at in vitro reviews, tissue tradition within the examine of pharmacologically lively brokers, and strategies of evaluate of drug job. The textual content additionally experiences invertebrate tissue and organ tradition in mobilephone examine; creation and techniques hired in plant tissue tradition; and development, differentiation and organogenesis in plant tissue and organ cultures.
The choice is a crucial resource of knowledge for readers attracted to the tradition of cells and tissues.

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Additional info for Cells and Tissues in Culture Methods, Biology and Physiology

Sample text

Green and Goldberg suggested that the increase in hydrogen-ion concentration might inhibit the synthesis of cellular protein, and hence mitosis, but that the increase of lactate ion might specifically stimulate collagen synthesis. Green and Hamerman (1964) noted also that the 3T6 line of cells was capable of synthesizing an hexuronic acid containing polysaccharide ; since both streptococcal and testicular hyaluronidase degraded the polysaccharide, they assumed it consisted essentially of hyaluronate.

G. FINE-STRUCTURE OF FIBROGENIC CELLS Certain features of the fine-structure of cells in culture have been described earlier in this chapter. The purpose now is to discuss briefly certain points in the cytoplasmic morphology of those cells in culture which are held to be directly concerned in the production of intercellular material. Steams (1940) reported the presence of cytoplasmic granules in fibroblasts of healing wounds in the ears of rabbits and suggested they were directly concerned in the formation of collagen fibres.

They suggested that the sulphate was being used in the assembly of macromolecular components of the ground substance ; that the non-collagenous protein is probably synthesized before the polysaccharide moiety of the chondroitin sulphate-protein complex is indicated by the work of Telser, Robinson 1. MOLECULAR ORGANIZATION OF CELLS AND TISSUES 47 and Dorfman (1965). At present, therefore, the balance of evidence favours the view that the Golgi zone is not directly concerned in either a definitive step in the synthesis of collagen, or in the transport of collagenous protein through the cell.

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