By Cliff L Hedley
The compounds making up the dry weight of seeds play an important position in identifying the dietary caliber and finally the makes use of and fiscal worth of the seed. The carbohydrate fraction may be divided into starch, fiber and soluble carbohydrates.The results of an enormous collaborative application throughout Europe, this booklet covers the chemistry, dietary price, body structure, processing and breeding/biotechnology of carbohydrates in grain legume seeds. there's at present no ebook to be had which covers the innovations for bettering carbohydrates in grain legumes (peas, beans etc.) which takes into consideration either dietary and agricultural requisites. This ebook fills that gap
* huge part overlaying the topical topic of genetic manipulation techniques
* info in the ebook comes in handy on an international large scale
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Extra resources for Carbohydrates in grain legume seeds : improving nutritional quality and agronomic characteristics
6. A wide range of stationary phases are available for columns to customize a method for a particular class of soluble carbohydrate. 7. GC equipment is more common in laboratories and tends to be cheaper to purchase. Disadvantages: 1. Preparing the TMS derivatization of -OH groups can be difficult, but is crucial for successful results. 2. The chemicals involved are toxic, hazardous and expensive and must be disposed of safely. 3. The multiple peaks produced by TMS derivatives of reducing sugars can interfere with peaks of free and methylated cyclitols.
E. content of sugar); µ an overall mean; αi the effect of extraction medium; βj the effect of temperature or extraction procedure and εijk a random variable. According to Johansen et al. 0 mg ml−1 extract, corresponding to an injected amount of 1–180 µg. 9940 for raffinose and stachyose respectively, both when calibrated on basis of height and area. 9997). , 1988). Rapid (10 min) method of determination of total RFOs: • RP-HPLC using the method of Kvasnidka et al. (1996), see section on IMP-HPLC (pp.
A supply of compressed gases is needed. The gas has to be clean, dry and pure to produce consistent results. 5. An analytical run can sometimes take 1 h to complete, although shorter run times are possible. High performance liquid chromatography (HPLC) The water extract of soluble carbohydrates from biological material, (containing an internal standard) should be cleaned before HPLC analysis to improve reliability and resolution, using one of the following procedures. SAMPLE CLEAN-UP 1. The extract is filtered through a Sep-Pak C18 cartridge, pre-wetted with methanol and pure water.