By Norman Kharasch
Read or Download Biochemical Aspects of Prostaglandins and Thromboxanes. Proceedings of the 1976 Intra-Science Research Foundation Symposium December 1–3, Santa Monica, California PDF
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Additional resources for Biochemical Aspects of Prostaglandins and Thromboxanes. Proceedings of the 1976 Intra-Science Research Foundation Symposium December 1–3, Santa Monica, California
Sci . 9 (1973) . Svensson , J. , Wakabayashi , T. , an d B. , Proc . Natl . Acad . Sci . USA, 71 , 34 5 Prostaglandi n Biosynthesi s 3. 4. 5. 6. 7. 8. 9. 10 . 11 . 12 . 13 . 14 . 15 . 16. 17 . 18 . 19 . 20. 21. 22 . 23. 13 Nugteren , D . Ç. , an d Hazelhof , Å. , Biochim . Biophys . Acta , 326 , 44 8 (1973) . Miyamoto , T. , Yamamoto , S. , an d Hayaishi , Ï. , Proc . Natl , Acad . Sci . USA, 71 , 364 5 (1974) . Miyamoto , Ô. , Ogino , Í. , Yamamoto , S. , an d Hayaishi , Ï. , J. Biol . Chem. , 251 , 262 9 (1976) .
O f th e tota l amoun t o f radioactivit y los t fro m th e cell s afte r incubatio n i n serum , abou t 46 %cam e fro m phosphatidylcholine , 42 % fro m phosphatidylethanolamine, an d 11 %fro m phosphatidyl serine . The radioactiv e compound s i n th e mediu m whe n th e cell s wer e culture d i n th e presenc e o f seru m wer e identified ; 73 % of th e radioactivit y wa s foun d i n th e fre e arachidoni c aci d fraction ; 11 %an d 2 %ha d bee n converte d t o PGE2 an d PGF2 , respectively .
Fig . 11 . Inhibitio n o f th e releas e o f radioactivit y fro m [%]arachidoni c acid-labele d cell s b y actinomyci n D an d cycloheximide . Radioactively-labele d cell s wer e incubate d wit h 2 ml o f serum-supplemente d mediu m (ï) , o r wit h sam e mediu m containin g 4 y g actinomyci n D (Ä) , o r 2 0 y g cyclo heximid e (•) . Aliquot s o f 5 0 y l wer e withdraw n a t th e indicate d time s an d counte d fo r radioactivity . observe d whe n immunoreactiv e prostaglandin s wer e measured , inhibitio n b y actinomyci n D wa s delaye d 4 0 minutes , wherea s th e effec t o f cycloheximid e wa s faster .